文章基本信息

标题：Kinetics of conformational fluctuations in DNA hairpin-loops
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作者：Grégoire Bonnet ; Oleg Krichevsky ; Albert Libchaber 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：15
页码：8602-8606
DOI：10.1073/pnas.95.15.8602
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The kinetics of DNA hairpin-loop fluctuations has been investigated by using a combination of fluorescence energy transfer and fluorescence correlation spectroscopy. We measure the chemical rates and the activation energies associated with the opening and the closing of the hairpin for different sizes and sequences of the loop and for various salt concentrations. The rate of unzipping of the hairpin stem is essentially independent of the characteristics of the loop, whereas the rate of closing varies greatly with the loop length and sequence. The closing rate scales with the loop length, with an exponent 2.6 {+/-} 0.3. The closing rate is increased at higher salt concentrations. For hairpin closing, a loop of adenosine repeats leads to smaller rates and higher activation energies than a loop with thymine repeats.
关键词：molecular beacons/fluorescence correlation spectroscopy/folding kinetics/polymer conformation/fluorescence energy transfer