文章基本信息

标题：Characterizing semilocal motions in proteins by NMR relaxation studies
本地全文：下载
作者：Mark W. F. Fischer ; Lei Zeng ; Ananya Majumdar 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：14
页码：8016-8019
DOI：10.1073/pnas.95.14.8016
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The understanding of protein function is incomplete without the study of protein dynamics. NMR spectroscopy is valuable for probing nanosecond and picosecond dynamics via relaxation studies. The use of 15N relaxation to study backbone dynamics has become virtually standard. Here, we propose to measure the relaxation of additional nuclei on each peptide plane allowing for the observation of anisotropic local motions. This allows the nature of local motions to be characterized in proteins. As an example, semilocal rotational motion was detected for part of a helix of the protein Escherichia coli flavodoxin.