文章基本信息

标题：Functional cleavage of the common cytokine receptor γ chain (γc) by calpain
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作者：Masayuki Noguchi ; Apurva Sarin ; M. Javad Aman 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1997
卷号：94
期号：21
页码：11534-11539
DOI：10.1073/pnas.94.21.11534
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The small subunit of calpain, a calcium-dependent cysteine protease, was found to interact with the cytoplasmic domain of the common cytokine receptor {gamma} chain ({gamma}c) in a yeast two-hybrid interaction trap assay. This interaction was functional as demonstrated by the ability of calpain to cleave in vitro-translated wild-type {gamma}c, but not {gamma}c containing a mutation in the PEST (proline, glutamate, serine, and threonine) sequence in its cytoplasmic domain, as well as by the ability of endogenous calpain to mediate cleavage of {gamma}c in a calcium-dependent fashion. In T cell receptor-stimulated murine thymocytes, calpain inhibitors decreased cleavage of {gamma}c. Moreover, in single positive CD4+ thymocytes, not only did a calpain inhibitor augment CD3-induced proliferation, but antibodies to {gamma}c blocked this effect. Finally, treatment of cells with ionomycin could inhibit interleukin 2-induced STAT protein activation, but this inhibition could be reversed by calpain inhibitors. Together, these data suggest that calpain-mediated cleavage of {gamma}c represents a mechanism by which {gamma}c-dependent signaling can be controlled.