文章基本信息

标题：Plasma membrane inositol 1,4,5-trisphosphate receptor of lymphocytes: selective enrichment in sialic acid and unique binding specificity
本地全文：下载
作者：A A Khan ; J P Steiner ; S H Snyder 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1992
卷号：89
期号：7
页码：2849-2853
DOI：10.1073/pnas.89.7.2849
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The inositol 1,4,5-trisphosphate receptor (IP3R) associated with plasma membranes of lymphocytes differs in terminal sugar content and binding specificity from the cerebellar receptor, which is localized to endoplasmic reticulum. Lectin column chromatography reveals that 30% of IP3R in the thymus contains sialic acid, reflecting a plasma membrane association, in contrast to 5% of cerebellar IP3R. IP3R in thymus and plasma membrane fractions of Jurkat lymphocytes differs from IP3R of Jurkat microsomes and cerebellum in inositol phosphate specificity. The plasma membrane IP3R has lower affinity for IP3 but higher affinity for inositol 1,3,4,5-tetrakisphosphate, which may reflect a unique regulation of calcium at the plasma membrane by inositol phosphates.