首页    期刊浏览 2024年12月13日 星期五
登录注册

文章基本信息

  • 标题:Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts.
  • 本地全文:下载
  • 作者:M Nagy ; F Lacroute ; D Thomas
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1992
  • 卷号:89
  • 期号:19
  • 页码:8966-8970
  • DOI:10.1073/pnas.89.19.8966
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A cDNA encoding the dihydroorotate dehydrogenase (DHOdehase; EC 1.3.3.1 ) of the yeast Schizosaccharomyces pombe was isolated by functional complementation in Saccharomyces cerevisiae. A divergent subcellular compartmentation of the DHOdehase of each yeast was shown. The DHOdehase from Sch. pombe was localized in the mitochondria whereas its homolog from S. cerevisiae was found to be cytosolic. The heterologous expression of the Sch. pombe enzyme in S. cerevisiae allowed us to demonstrate that the Sch. pombe DHOdehase activity requires the integrity of the mitochondrial electron transport chain. Indeed, the presence of a mutation inactivating cytochrome b abolished the complementation of a S. cerevisiae ura1 mutant by the corresponding Sch. pombe gene. By contrast, in vitro studies have revealed that the DHOdehase of S. cerevisiae uses fumarate as terminal electron acceptor. These results are discussed in relation to the anaerobic growth competence of the two yeasts and to the fermentative processes they use.
国家哲学社会科学文献中心版权所有