文章基本信息

标题：Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy.
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作者：P C Van Zijl ; C T Moonen ; P Faustino 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1991
卷号：88
期号：8
页码：3228-3232
DOI：10.1073/pnas.88.8.3228
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A method is outlined that completely separates intracellular and extracellular information in NMR spectra of perfused cells. The technique uses diffusion weighting to exploit differences in motional properties between intra- and extracellular constituents. This allows monitoring of intracellular metabolism, and of transport of small drugs and nutrients through the cell membrane, under controlled physiological conditions. As a first example, proton spectra of drug-resistant MCF-7 human breast cancer cells are studied, and uptake of phenylalanine is monitored.