文章基本信息

标题：Microtubule mediation of cytoplasmic and nuclear maturation during the early stages of resumed meiosis in cultured mouse oocytes
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作者：J Van Blerkom
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1991
卷号：88
期号：11
页码：5031-5035
DOI：10.1073/pnas.88.11.5031
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A perinuclear accumulation of mitochondria characterizes the premetaphase I stages of reinitiated meiosis in the laboratory mouse oocyte. The cellular basis of this organelle-specific translocation was examined by fluorescent probe analysis, immunostaining, and immunoelectron microscopy in oocytes cultured at specific stages of meiotic maturation in the presence and absence of drugs that influence the stability of microfilaments and microtubules. The results suggest that a temporal, spatial, and developmental relationship exists between the location of microtubule organizing centers and the progressive translocation of mitochondria to the nuclear region. The findings indicate that mitochondrial translocations are mediated by microtubules and that individual microtubule organizing centers are not only foci for mitochondrial aggregation but may also facilitate the establishment of the circular bivalent configuration.