文章基本信息

标题：Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats
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作者：A L Kierszenbaum ; O Lea ; P Petrusz 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：3
页码：1675-1679
DOI：10.1073/pnas.78.3.1675
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A method for the isolation and culture of epididymal epithelial cells obtained from pubertal and old adult rats is described. This method permits the establishment of primary cultures of these cells in monolayers from aggregates isolated from whole epididymides and major epididymal anatomical segments (caput, corpus, and cauda) after trypsin and collagenase digestions. A large number of cultured epididymal cells retain a differentiated function as demonstrated by the immunocytochemical and radioimmunoassay finding of acidic epididymal glycoprotein, a spermatozoa-coating protein secreted by the principal cells of rat epididymis. The proliferative potential of cultured epididymal cells obtained from pubertal and old adult donors can be documented by [3H]thymidine labeling and mitotic indices without significant loss of gene expression for acidic epididymal glycoprotein. Results of this study demonstrate that epididymal epithelial cells, consisting of a predominant population of principal cells, can be isolated, cultured, and maintained for up to 3 months.