文章基本信息

标题：Mapping a eukaryotic promoter: a DNA sequence required for in vivo expression of adenovirus pre-early functions
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作者：T F Osborne ; R E Schell ; E Burch-Jaffe 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：3
页码：1381-1385
DOI：10.1073/pnas.78.3.1381
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：This study defines a DNA sequence upstream from the mRNA cap site required for in vivo expression of the adenovirus 2 pre-early region. The adenovirus 2 pre-early region and flanking sequences were cloned in Escherichia coli plasmid pBR322. Derivatives of the plasmid lacking portions of the upstream viral DNA sequence were constructed. An assay was devised to test the ability of these plasmid DNAs to complement an adenovirus 5 mutant with a deletion in the pre-early region. Plasmids that retained at least 38 base pairs upstream from the mRNA cap site had complementing activity similar to that of the original plasmid, which contains 229 base pairs of upstream viral sequence. However, plasmids retaining 23 or fewer base pairs of viral sequence upstream from the cap site had significantly reduced complementing activity. These results indicate that a portion of the adenovirus 2 sequence between 23 and 38 base pairs upstream from the mRNA cap site is required for expression of the pre-early region. This interval includes the Goldberg-Hogness box-like sequence T-A-T-T-T-A-T-A.