文章基本信息

标题：Enzymatic hydrolysis of cellulose: Visual characterization of the process
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作者：Alan R. White ; R. Malcolm Brown
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：2
页码：1047-1051
DOI：10.1073/pnas.78.2.1047
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cellulose from the Gram-negative bacterium Acetobacter xylinum has been used as a model substrate for visualizing the action of cellulase enzymes from the fungus Trichoderma reesei. High-resolution electron microscopy reveals that A. xylinum normally produces a ribbon of cellulose that is a composite of bundles of crystalline microfibrils. Visual patterns of the process of cellulose degradation have been established. Enzymes are initially observed bound to the cellulose ribbon. Within 10 min, the ribbon is split along its long axis into bundles of microfibrils which are subsequently thinned until they are completely dissolved within 30 min. Incubations with purified components of the cellulase enzyme system produced less dramatic changes in ribbon structure. Purified 1,4-{beta}-D-glucan cellobiohydrolase I (D) (EC 3.2.1.91 ) produced no visible change in cellulose structure. Purified endo-1,4-{beta}-D-glucanase IV (EC 3.2.1.4 ) produced some splaying of ribbons into microfibril bundles. In both cases, whole ribbons were present even after 60 min of incubation, visually confirming the synergistic mode of action of these enzymes.
关键词：Trichoderma reesei ; Acetobacter xylinum ; high-resolution electron microscopy ; endoglucanase ; cellobiohydrolase