文章基本信息

标题：Detection of enzyme polymorphism by using monoclonal antibodies
本地全文：下载
作者：C A Slaughter ; M C Coseo ; M P Cancro 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：2
页码：1124-1128
DOI：10.1073/pnas.78.2.1124
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Six monoclonal antibodies against human placental alkaline phosphate [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1 ], a highly polymorphic enzyme, were tested for reactivity against a panel of 295 placental extracts that had been typed electrophoretically. The products of the three common alleles as well as several rare alleles could be discriminated by the various antibodies. In some cases differences between allelic products were reflected by essentially "all-or-none" reactions, but in other cases the differences were smaller and demonstrable only by quantitative analysis of the binding. Evidence for allelic differences not detectable electrophoretically was also obtained.