文章基本信息

标题：Comparison of [3H]glucosamine-labeled glycoproteins from human renal cancer and normal kidney epithelial cell cultures by two-dimensional polyacrylamide gel electrophoresis
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作者：S Ogata ; R Ueda ; K O Lloyd 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1981
卷号：78
期号：2
页码：770-774
DOI：10.1073/pnas.78.2.770
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Two-dimensional polyacrylamide gel electrophoresis has been used to compare the Nonidet P-40 soluble, [3H]glucosamine-labeled glycoproteins of human kidney cancer cell lines and short-term cultures of normal kidney epithelia. Two pairs of autologous combinations of tumor and normal cells and also six other cancer lines and four normal cultures were examined. Because autologous cells could be compared, possible differences due to allogeneic variations could be eliminated. The electrophoretic patterns given by tumor and normal cells were extremely similar. Nevertheless, more than 10 differences could be recognized when randomly selected combinations of cancer and normal cells were examined in detail. Of these differences, however, only one was consistently found in all possible combinations of cancer and normal cells. Cancer cells had three prominent components of molecular weight 27,500 and pIs of 5.7, 5.3, and 4.9, respectively. Normal cells have a fainter and different constellation of spots in this region, with the more acid component (pI 4.9) being absent (or barely detectable). The pattern of fetal kidney cells resembled that given by normal adult kidney.