文章基本信息

标题：Control of gene expression by a mobile recombinational switch
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作者：D E Berg
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：8
页码：4880-4884
DOI：10.1073/pnas.77.8.4880
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Transposable recombinational switches may play important roles in the evolution of bacterial populations by increasing flexibility in the control of expression of particular genes and thereby maintaining heterogeneity in clones of cells growing in a uniform environment. Experiments reported here show that Tn5-112, a deletion derivative of kanamycin-resistance transposon Tn5, can function as such a mobile recombinational switch. The internal deletion in Tn5-112 removes transcription termination signals and permits transcription initiated within the element to continue into nearby bacterial genes. Consequently, in one orientation Tn5-112 stimulates distal gene expression, whereas in the other orientation the normal polarity imposed by wild-type Tn5 intervenes and distal gene expression is not stimulated. Because Tn5-112 contains terminal inverted repeats, intramolecular recombination can invert the Tn5-112 element and alter gene expression. Tn5-112 is transposition deficient. Its mobility derives from the recessive nature of the transposition deficiency and, in this study, from the possibility of homologous recombination which permits its placement in either orientation at any site occupied by nother Tn5 element.