文章基本信息

标题：Inhibition of human polymorphonuclear leukocyte chemotaxis by oxygenated sterol compounds
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作者：L I Gordon ; J Bass ; S Yachnin 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：7
页码：4313-4316
DOI：10.1073/pnas.77.7.4313
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：When preincubated with certain oxygenated sterol compounds in lipoprotein-depleted serum [20% (vol/vol)], human polymorphonuclear leukocytes show inhibition of chemotaxis toward the synthetic dipeptide N-formylmethionylphenylalanine without alteration of random movement or loss of cell viability. These effects can occur at sterol concentrations as low as 6.25 microM and after as little as 5 min of preincubation, but they are increased at higher concentrations and longer preincubation times. The inhibition can be almost completely reversed by preincubation in lipoprotein-replete serum [human AB serum, 20% (vol/vol)] and may be partially corrected by addition of free cholesterol (0.125 mM) to the medium. These effects are unlikely to be due to inhibition of cellular sterol synthesis, competition for chemotaxin membrane binding sites, or deactivation of the leukocytes but they may be a consequence of insertion of the sterol molecule into the leukocyte plasma membranes.