文章基本信息

标题：Prostaglandin synthesis by macrophages requires a specific receptor-ligand interaction
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作者：C A Rouzer ; W A Scott ; J Kempe 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：7
页码：4279-4282
DOI：10.1073/pnas.77.7.4279
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The ingestion of particles by macrophages leads to the prompt induction of prostaglandin (PG) synthesis. We have now dissected the endocytic process and examined the requirements of prostaglandin E (PGE) synthesis for particle attachment, membrane interiorization, and phagosome-lysosome fusion. Macrophages that were loaded with the polyanion dextran sulfate and exhibited a greater than 99% inhibition of phagosome-lysosome fusion produced normal amounts of PGE upon challenge with zymosan. Inhibition of membrane interiorization with cytochalasin D was similarly ineffective in blocking PGE synthesis. The addition of large numbers of unmodified polystyrene latex beads, which were readily ingested by macrophages, failed to stimulate PGE synthesis. However, when macrophages were challenged with latex beads coated with immune complexes, an increased synthesis of PGE resulted. No response occurred if the complex was prepared with the F(ab')2 fragment of IgG. Similar results occurred when nonphagocytizable Sephadex beads coated with immune complexes were employed. We conclude that particle binding to the Fc receptor of the macrophage plasma membrane is a sufficient stimulus for PGE synthesis.