文章基本信息

标题：Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression
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作者：C Benoist ; P Chambon
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：7
页码：3865-3869
DOI：10.1073/pnas.77.7.3865
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A recombinant plasmid was constructed by insertion of the early genes of simian virus 40 (SV40) into pBR322. When it was introduced into eukaryotic cells, the SV40 early genes were expressed. We have made deletion mutants of this plasmid, from which the major cap sites of SV40 early mRNAs have been removed along with some of the sequences upstream. The deleted sequences appear to be dispensable for early gene expression, but this does not necessarily imply that they serve no function in the initiation of transcription on wild-type SV40.