文章基本信息

标题：Effect of bleaching light on measurements of lateral diffusion in cell membranes by the fluorescence photobleaching recovery method
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作者：D E Wolf ; M Edidin ; P R Dragsten 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：4
页码：2043-2045
DOI：10.1073/pnas.77.4.2043
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The Fc receptors for IgE on rat basophil leukemia 2H3 cells were labeled with either rhodamine- or fluorescein-conjugated IgE and then with Fab fragments of anti-IgE conjugated with the other dye. Fluorescence photobleaching recovery measurements of lateral diffusion were performed with one of the dyes before and after extensive bleaching of the other dye over an entire cell. Bleaching one dye did not affect subsequent measurements made with the other dye. We thus detect no evidence for photoinduced artifacts in fluorescence photobleaching recovery experiments.