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标题：Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus
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作者：Georg Krohne ; Werner W. Franke
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：2
页码：1034-1038
DOI：10.1073/pnas.77.2.1034
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Nuclei of vitellogenic oocytes of the frog, Xenopus laevis, contain a prominent protein, representing about 10% of nuclear protein, which is characterized by a polypeptide of Mr 30,000. This protein is highly phosphorylated and acidic, displays several isoelectric variants with pI values ranging from 4.7 to 5.3, shows a high thermostability, is not stably complexed with other proteins, and is readily extracted in buffer solutions. Guinea pig antibodies against this protein have allowed its specific immunoprecipitation and localization by immunofluorescence microscopy, using both frozen tissue sections and cells grown in vitro. The protein is located almost exclusively in the nucleus where it appears to be spread throughout the nuclear interior. It is also a major nucleus-specific protein in vitellogenic and previtellogenic oocytes of other amphibian species as well as in other cell types, including hepatocytes, follicle epithelial cells, and cultured Xenopus cells, but is not detected in nuclei of transcriptionally inactive cells such as erythrocytes and spermatids. An immunologically related, if not identical, protein occurs in nuclei of higher vertebrate cells, including mammals. The properties of this abundant nuclear phosphoprotein and its possible relationship to the "nucleosome assembly factor" protein are discussed. It is suggested that this soluble protein serves a general nuclear function.
关键词：phosphoproteins ; nonhistones ; nuclear sap ; nucleocytoplasmic compartmentation ; immunofluorescence microscopy