文章基本信息

标题：Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens
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作者：M. F. Thomashow ; R. Nutter ; K. Postle 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：11
页码：6448-6452
DOI：10.1073/pnas.77.11.6448
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The Ti plasmid sequences (T-DNA) from the octopine-producing crown gall tumor A6S/2 were isolated by molecular cloning, using the bacteriophage {lambda} vector Charon 4A. Analysis of the cloned DNA segments indicates that the Ti plasmid sequences are covalently joined to plant nuclear DNA. These data demonstrate that genetic recombination between a eukaryote and a prokaryote can occur as a natural phenomenon.
关键词：genomic DNA cloning ; crown gall tumors