文章基本信息

标题：Immunological identification of the human erythrocyte glucose transporter
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作者：D C Sogin ; P C Hinkle
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：10
页码：5725-5729
DOI：10.1073/pnas.77.10.5725
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A rabbit antibody against the human erythrocyte glucose transporter was purified by affinity chromatography and used to determine the distribution of transporter on polyacrylamide gels after electrophoresis in sodium dodecyl sulfate. Fresh erythrocyte ghosts showed transporter only at the broad 55,000 Mr band, as did the isolated transporter. HeLa cell plasma membranes showed a similar band of crossreacting material at Mr 55,000. The amount of crossreacting material in human erythrocyte ghosts and in plasma membranes from human HeLa cells and mouse L-1210 cells was determined in an enzyme-linked immunosorbent assay which gave results consistent with the extent of glucose-reversible binding of cytochalasin B.