文章基本信息

标题：Method for preparing cultures of central neurons: cytochemical and immunochemical studies
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作者：J Sotelo ; C J Gibbs ; D C Gajdusek 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1980
卷号：77
期号：1
页码：653-657
DOI：10.1073/pnas.77.1.653
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We report a simplified method for culturing fetal central nervous system cells predominantly inducing neurons that grow, differentiate, and live in vitro for as long as 10 weeks. These central nervous system cells form a confluent cell culture in which about 80% of the cells are fully differentiated neurons producing interconnecting axons and dendrite processes and live upon a sparse underlying population of fibrillary and protoplasmic astrocytes, oligodendrocytes, and fibroblasts. Morphological and cytochemical characteristics of these cell types, based on immunofluorescent cell specific markers and silver staining of neurons, are presented.