文章基本信息

标题：Iron—dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy
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作者：Anne H. Dutton ; K. T. Tokuyasu ; S. J. Singer 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1979
卷号：76
期号：7
页码：3392-3396
DOI：10.1073/pnas.76.7.3392
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron--dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil--antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin--antibody conjugates.
关键词：cell ultrastructure ; Imposil ; ferritin ; ultracryotomy