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  • 标题:Functional reconstitution of photosynthetic reaction centers in planar lipid bilayers
  • 本地全文:下载
  • 作者:M. Schönfeld ; M. Montal ; G. Feher
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1979
  • 卷号:76
  • 期号:12
  • 页码:6351-6355
  • DOI:10.1073/pnas.76.12.6351
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Planar lipid bilayers containing reaction centers from Rhodopseudomonas sphaeroides R-26 were formed by apposing two reaction center--lipid monolayers formed from a reaction center--lipid complex in hexane. Secondary donors (cytochrome c) and acceptors (ubiquinone-0) were added on opposite sides of the membrane. Upon illumination, this system generated transient and steady-state voltages and currents. The wavelength dependence of the photoresponse matched the absorption spectrum of reaction centers. A simple model based on the transfer of charges across the membrane that explains the salient features of the photoresponse is presented.
  • 关键词:photosynthesis ; membrane reconstitution ; chemosmotic energy coupling ; transmembrane electron transfer
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