文章基本信息

标题：Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro
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作者：M S Horwitz
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：9
页码：4291-4295
DOI：10.1073/pnas.75.9.4291
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.