文章基本信息

标题：Mechanisms of antibody diversity: Multiple genes encode structurally related mouse κ variable regions
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作者：David J. McKean ; Michael Bell ; Michael Potter 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：8
页码：3913-3917
DOI：10.1073/pnas.75.8.3913
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The complete amino acid sequences of the variable regions of three mouse V{kappa}-21 kappa chains (A22, T111, and CB101) and one partial sequence (B32) have been determined and are compared to four previously reported V{kappa}-21 variable regions. These eight {kappa} variable region sequences have, with the exception of an amide difference at residue 1, identical amino-terminal 23-residue sequences, all are of the same length, and all have extensive amino acid sequence homology throughout the variable region. When these eight variable regions are grouped by sequence homology, five different groups (V{kappa}-21A, B, C, D, and E) are present whose members share common sets of amino acids within a group. Three groups of similar homology each contains at least two members (M63 and AB22 in V{kappa}-21B; M321 and T124 in V{kappa}-21C; and M70 and B32 in V{kappa}-21A). The repetition of these five characteristic subgroup sequences in this relatively small sample indicates that these subgroups are isotypes which are controlled by separate germline genes. It is unlikely that these sequences could have been randomly somatically generated in different animals from a single germ-line gene (parallel mutation). Although a limited number of comparisons are available, the sequence differences within the V{kappa}-21A, B, and C isotypes are limited to complementarity-determining regions and may have resulted from somatic mutations. The {kappa} chains comprising the V{kappa}-21 isotypes offer a unique opportunity to compare the genetic interpretations of the primary amino acid sequence data with the nucleic acid hybridization data.
关键词：amino acid sequence ; genetics of antibody diversity ; Vκ-21 isotype