文章基本信息

标题：Chromosomal localization of human β globin gene on human chromosome 11 in somatic cell hybrids
本地全文：下载
作者：Albert Deisseroth ; Arthur Nienhuis ; Jeanne Lawrence 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：3
页码：1456-1460
DOI：10.1073/pnas.75.3.1456
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have successfully used a DNA{middle dot}cDNA molecular hybridization assay to directly determine the presence or absence of human {beta} globin gene sequences in 20 human--mouse somatic cell hybrids, each of which contained a different subset of human chromosomes. The assay is specific for the individual human globin genes and will detect the presence of a globin gene if the relevant chromosome is present in only 10% of the cells of a hybrid population. The content of human chromosomes in each hybrid clone was characterized by Giemsa 11 staining, Giemsa trypsin-Hoechst 33258 staining, and by the use of 22 independent isozyme markers for 17 different human chromosomes. All human chromosomes were present in one or more cell lines devoid of the human {beta} globin gene except for 6, 8, 9, 11, and 13. Among these latter chromosomes, only chromosome 11 was present in the six hybrid clones that contained the human {beta} globin gene. In fact, chromosome 11 was the only human chromosome that was present in all of the six hybrid clones found to be positive for the human {beta} globin gene. Two sister clones, 157-BNPT-1 and 157-BNPT-4, had similar subsets of human chromosomes except that 11 was present only in 157-BNPT-4. 157-BNPT-4 contained the human {beta} globin gene while 157-BNPT-1 did not. DNA from three hybrid lines was also annealed to purified human {gamma} globin cDNA; two lines positive for human {beta} globin gene sequences also contained human {gamma} globin gene sequences while one line was negative for both {beta} and {gamma} gene sequences. On the basis of these results, the human {beta} and {gamma} globin genes have been assigned to human chromosome 11.
关键词：gene mapping ; cloning ; cDNA ; fibroblasts ; molecular hybridization