文章基本信息

标题：ATP-dependent Ca2+ uptake into plant membrane vesicles
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作者：Joachim Gross ; Dieter Marmé
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：3
页码：1232-1236
DOI：10.1073/pnas.75.3.1232
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Membrane vesicles were extracted from etiolated and light-grown plants, a plant cell suspension culture, and an alga. Upon addition of ATP and Mg2+, active Ca2+ uptake into the vesicles against a concentration gradient was shown. The dependence of this uptake on ATP and Mg2+ concentrations, pH, and temperature is described. In the absence of oxalate, equilibrium between Ca2+ uptake and efflux was reached after about 30 min. In the presence of oxalate, Ca2+ accumulation continued for at least 120 min. Ca2+ efflux from preloaded vesicles did not depend on ATP. Addition of the ionophores A23187 and Ro 20-0006/006 caused an immediate release of accumulated free Ca2+. ATP-dependent Ca2+ uptake was not inhibited by 10 {micro}M oligomycin.
关键词：nucleotide specificity ; ionophores ; oxalate ; Mg²⁺-ATPase ; adenylate kinase