文章基本信息

标题：Association of cyclic GMP with gene expression of polytene chromosomes of Drosophila melanogaster
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作者：W A Spruill ; D R Hurwitz ; J C Lucchesi 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：3
页码：1480-1484
DOI：10.1073/pnas.75.3.1480
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The distribution of cyclic nucleotides on polytene chromosomes isolated from Drosophilia melanogaster salivary glands was examined by using an indirect immunofluorescent technique. With a fixative that minimized the loss of chromosomal proteins, cyclic GMP, but not cyclic AMP, was observed distributed along the chromosomes. The subchromosomal distribution of cyclic GMP correlated with genetically active sites on the chromosomes. After heat-shock treatments, the intensity of cyclic GMP fluorescence was markedly enhanced at specific loci on the chromosomes, with locus 93D as the most intensely fluorescent. Autoradiographic analysis with [3H]uridine revealed that 93D was the most transcriptionally active locus within a particular nucleus. These observations suggest that cyclic GMP may participate in processes associated with transcription on polytene chromosomes. The involvement of cyclic GMP in nuclear events associated with gene expression is discussed.