文章基本信息

标题：The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor
本地全文：下载
作者：S M Tilghman ; P J Curtis ; D C Tiemeier 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：3
页码：1309-1313
DOI：10.1073/pnas.75.3.1309
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mouse beta-globin in encoded in a discontinuous structural gene interrupted by a 550-base pair intervening sequence of DNA. Correspondingly, the mature beta-globin mRNA appears to be synthesized via a 15S precursor, the length of which roughly equals the total length of the coding and intervening sequences of the beta-globin gene. Using the electron microscope to visualize hybrid structures formed between this gene and the purified 15S beta-globin mRNA precursor, we show that the intervening sequence is present within the larger precursor molecule. This finding suggests that the precursor mRNA is processed through the removal and rejoining of internal RNA sequences.