文章基本信息

标题：An amino-terminal fragment of lac repressor binds specifically to lac operator
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作者：Ronald T. Ogata ; Walter Gilbert
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：12
页码：5851-5854
DOI：10.1073/pnas.75.12.5851
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have examined the effect of an aminoterminal peptide from the lac repressor (residues 1-59) on the methylation of purines in the lac operator with dimethyl sulfate. The peptide perturbs the methylation of the operator, and the peptide-induced pattern of inhibition and enhancement of methylation, across the operator, closely resembles the pattern induced by intact repressor. This demonstrates that this small amino-terminal peptide binds specifically to the lac operator and that the mechanism of recognition and binding is basically the same as that of intact repressor.
关键词：dimethyl sulfate methylation ; tryptic peptide ; bromouracil DNA ; protein domains