文章基本信息

标题：Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor
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作者：L P Guarente ; J Beckwith
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：1
页码：294-297
DOI：10.1073/pnas.75.1.294
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have isolated a rifampicin-resistant mutant of Escherichia coli RNA polymerase that restores transcription termination in strains with a defective rho protein. In such strains, the mutant RNA polymerase terminates transcription at normally rho-dependent sites at the end of the trp operon, in bacteriophage lambda, and within the lac operon. In addition, a strain with this mutant RNA polymerase remains viable with an amber mutation in rho, whereas a strain with wild-type RNA polymerase does not. These results suggest that the mutant RNA polymerase can terminate transcription at normally rho-dependent sites in the absence of rho.