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标题：Specific changes in the collagen phenotype of BALB 3T3 cells as a result of transformation by sarcoma viruses or a chemical carcinogen
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作者：Ryu-ichiro Hata ; Beverly Peterkofsky
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：7
页码：2933-2937
DOI：10.1073/pnas.74.7.2933
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Radioactive proline-labeled procollagen, accumulated during a 3-hr incubation of normal and transformed BALB 3T3 cultures, was treated with pepsin and the resulting collagen components were analyzed by carboxymethyl-cellulose chromatography and sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence or absence of reducing agent. Collagen in the medium of three subclones of BALB 3T3 A-31 that exhibited contact-inhibition of growth at confluence, as well as in the medium of one that did not, consisted of 1 and 2 subunits in the ratio of 3:1, suggesting that 3T3 cells synthesize type I collagen, [1(I)]22, and another type, which we have designated X, composed of 1 chains, which may or may not be identical to 1(I). Culture medium from 3T3 transformed by Kirsten or Moloney sarcoma virus contained type I collagen and another type differing from I and X and designated as type Y. The latter appeared to be similar to type III collagen [1(III)]3, since it contained intrahelical disulfide bonds. Analysis of intracellular collagen also demonstrated the presence of type III in Ki-3T3 and its absence from 3T3 cells. Collagen components from the medium of a simian virus 40 transformant were identical to those of the contact-inhibited clones, while the collagen from a 4-nitroquinoline-1-oxide-induced transformant was composed mainly of two components differing from 1(I), 2, or 1(III). These results suggest that the type of collagen accumulated in transformed cell cultures may be specifically related to the transforming agent.
关键词：collagen types ; 4-nitroquinoline-1-oxide ; simian virus 40