文章基本信息

标题：Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields
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作者：J C Myers ; S Spiegelman ; D L Kacian 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：7
页码：2840-2843
DOI：10.1073/pnas.74.7.2840
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight of 2.6 X 10(6), equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.