文章基本信息

标题：Molecular weight of bacteriorhodopsin solubilized in Triton X-100
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作者：J A Reyenolds ; W Stoeckenius
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：7
页码：2803-2804
DOI：10.1073/pnas.74.7.2803
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin is monomeric under the conditions of solubilization with a molecular weight of 24,250+/-2,000 and binds approximately one micelle of Triton X-100.