文章基本信息

标题：Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase
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作者：Donald M. Coen ; John R. Bedbrook ; Lawrence Bogorad 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：12
页码：5487-5491
DOI：10.1073/pnas.74.12.5487
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In vitro linked transcription-translation of chloroplast DNA has been used to show that the large subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39 ] is encoded by Zea mays chloroplast DNA. A BamHI-generated chloroplast DNA sequence cloned in Escherichia coli is shown to direct the in vitro synthesis of this protein identified as large subunit by its size, serological properties, and limited proteolytic digestion products.
关键词：molecular cloning ; restriction fragments ; linked transcription-translation