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  • 标题:mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu
  • 本地全文:下载
  • 作者:F Chu ; P Caldwell ; H Weissbach
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1977
  • 卷号:74
  • 期号:12
  • 页码:5387-5391
  • DOI:10.1073/pnas.74.12.5387
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:RNA extracted from growing Escherichia coli can direct the in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu when an E. coli system is used. The synthesized L12 can be bound to L12-depleted ribosomes and the synthesized elongation factor Tu can form complexes with both elongation factor Ts and GDP. Guanosine 5'-diphosphate 3'-diphosphate has no effect on the synthesis of these proteins from an RNA template but inhibits their synthesis when a DNA template is used.
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