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标题：Antigen-specific purification of blocking factors from sera of mice with chemically induced tumors
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作者：J T Nepom ; I Hellström ; K E Hellström 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：10
页码：4605-4609
DOI：10.1073/pnas.74.10.4605
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Serum from mice with growing tumors can prevent ("block") the destruction of tumor cells by immune lymphocytes, as measured in a microcytotoxicity assay. Factors responsible for this blocking activity were purified by binding to immune adsorbents that had been prepared from antibodies obtained by immunizing BALB/c mice to the homologous tumors. Two transplantable BALB/c sarcoma lines with individually different tumor-specific transplantation antigens were studied in parallel. The original tumor-specific blocking activity was recovered by elution of the immune adsorbents; that is, (i) eluates blocked the reduction of surviving tumor cell targets by immune lymphocytes only if the tumor specificity, and (ii) immune adsorbent columns prepared from tumor-immune sera recognized the purified blocking fractions in a tumor-specific fashion, indicating that a portion of the humoral response in the immune mice was directed against a factor that was individually distinct for each tumor. Absorption of eluates with the homologous tumor cells removed their blocking activity, indicating that the blocking factors have antigen-binding properties. Blocking activity in the purified fractions resided in molecules presumptively identified as glycoproteins by affinity chromatography on concanavalin A-Sepharose.