文章基本信息

标题：Endonuclease recognition sites mapped on Zea mays chloroplast DNA
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作者：John R. Bedbrook ; Lawrence Bogorad
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：12
页码：4309-4313
DOI：10.1073/pnas.73.12.4309
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The closed-circular DNA molecules of 85 x 106 daltons from Zea mays chloroplasts were isolated, digested with the restriction endonucleases Sal I, Bam I, and EcoRI, and the resulting fragments sized by agarose gel electrophoresis. A map of maize chloroplast DNA showing the relative location of all the Sal I recognition sequences and many of the Bam I and EcoRI recognition sites was determined. A DNA sequence representing approximately 15% of the Zea mays chloroplast genome is repeated. The two copies of this sequence are in an inverted orientation with respect to one another and are separated by a nonhomologous sequence representing approximately 10% of the genome length. The inverted repeats contain the genes for chloroplast ribosomal RNAs.
关键词：chloroplast rRNAs ; Sal I ; Bam I ; Eco RI ; hybridization