文章基本信息

标题：Headgroup conformation and lipid--cholesterol association in phosphatidylcholine vesicles: a 31P(1H) nuclear Overhauser effect study
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作者：P L Yeagle ; W C Hutton ; C H Huang 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1975
卷号：72
期号：9
页码：3477-3481
DOI：10.1073/pnas.72.9.3477
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The nuclear Overhauser effect has been observed in the nuclear magnetic resonance spectra of 31P. The information content of the nuclear Overhauser effect has been applied to the structure and dynamic properties of phosphatidylcholine vesicles. In the vesicles only 1/3 of the theoretical maximum nuclear Overhauser effect enhancement is observed. This result is accounted for by dipolar interactions between the N-methyl protons and the phosphate of phosphatidylcholine, and a correlation time for internal motion of 1.4 X 10(-9) sec. Addition of up to 30% cholesterol does not change the nuclear Overhauser effect enhancement or spin-lattice relaxation time of the vesicles. It is argued that the OH group of cholesterol is hydrogen bonded to the ester carbonyl oxygen of the phosphatidylcholine molecules.