文章基本信息

标题：Relationship between release of surface proteins and metabolic activation of sea urchin eggs at fertilization
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作者：J D Johnson ; D Epel
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1975
卷号：72
期号：11
页码：4474-4478
DOI：10.1073/pnas.72.11.4474
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Macromolecular components are released from sea urchin eggs when their metabolism is activated at fertilization or by incubation in ammonia. When the released material is dialyzed, concentrated, and added back to partially activated eggs the rate of protein synthesis is suppressed to the level of the unactivated egg. The surface proteins of the unfertilized eggs can be labeled with 125I by a lactoperoxidase procedure. When fertilized or activated with various parthenogenetic agents, 15-25% of the total labeled protein is released; most of the label is associated with a 150,000-dalton glycoprotein. The extent of metabolic activation, as assessed by measuring increased protein synthesis, is correlated with the amount of surface label released. Several other proteins are released during activation but are not labeled by the lactoperoxidase procedure in the intact cell. We have not yet identified which of these components is responsible for suppressing protein synthesis, nor do we know if any of the other metabolic changes of fertilization such as K+ conductance and DNA synthesis are also suppressed. We suggest that these released components are surface molecules involved in maintaining the low metabolic state occurring at the end of oogenesis and that removal of these components during fertilization results in the release of the suppression of the egg.