文章基本信息

标题：Ferritin-Conjugated Antibodies Used for Labeling of Organelles Involved in the Cellular Synthesis and Transport of Procollagen
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作者：Bjørn R. Olsen ; Darwin J. Prockop
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：2033-2037
DOI：10.1073/pnas.71.5.2033
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：An improved procedure was used to conjugate ferritin to antibodies specific for the NH2-terminal extensions on the precursor form of collagen known as procollagen. The ferritin-antibody conjugates were then used to determine the localization of procollagen in fibroblasts isolated from chick-embryo tendons. Procollagen was found in the cisternae of the endoplasmic reticulum, indicating that the protein passes into this compartment early in its biosynthesis. Specific labeling of large Golgi vacuoles was also observed, suggesting that this compartment is also involved in the secretory process. When cells were incubated with colchicine so that the secretion of procollagen was delayed, there was an increase of large, smooth-surfaced vacuoles in the cells and these vacuoles were labeled with the ferritin-antibody conjugate.
关键词：ferritin-antibody conjugates ; Golgi vacuoles ; protein secretion