文章基本信息

标题：Isolation of the Tumor-Inducing RNA from Oncogenic and Nononcogenic Agrobacterium tumefaciens
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作者：M. Beljanski ; M. I. Aaron-Da Cunha ; M. S. Beljanski 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：1585-1589
DOI：10.1073/pnas.71.5.1585
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Two RNA fractions have been isolated and purified from both oncogenic and nononcogenic strains of Agrobacterium tumefaciens. Both RNAs are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of Datura stramonium plants. One of these RNA fractions was found to be bound to an RNA-directed DNA polymerase, while the other was associated with the bacterial DNA. Physical evidence suggests that both are single stranded and small in size; linear sucrose gradients show that their size corresponds to a value of 5-6 S. A concentration of 4-5 {micro}g of the RNAs dissolved in 0.01 ml of water is effective in initiating the formation of transplantable tumors in Datura plants.
关键词：crown gall disease ; bacterial RNA ; transplantable tumors