文章基本信息

标题：Novel Properties of Satellite DNA from Muskmelon
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作者：Arnold J. Bendich ; Robert S. Anderson
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：4
页码：1511-1515
DOI：10.1073/pnas.71.4.1511
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The purified dense satellite of muskmelon (Cucumis melo L.) represents 30% of the total DNA and forms a sharp, unimodal peak in both neutral and alkaline CsCl gradients. Spectrophotometric melting and DNA reassociation analyses revealed that one-third of the satellite is high melting and has a complexity of about 2.5 x 105 daltons, while the remaining two-thirds of the satellite melts 8{degrees} lower and has a complexity of about 5 x 108 daltons. The thermal stability of reassociated satellite DNA indicates that the multiple copies of the two melting fractions are essentially identical. The sharp, unimodal peak in Cs2SO4 becomes two distinct peaks when either silver or mercuric ions are included in the Cs2SO4 gradient
关键词：melting curves ; CsCl gradients ; Ag⁺-Cs₂SO₄ gradients