文章基本信息

标题：Transfer RNA Conformation in Solution Investigated by Isotope Labeling
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作者：Ronald C. Gamble ; Paul R. Schimmel
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：4
页码：1356-1360
DOI：10.1073/pnas.71.4.1356
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The incorporation of tritium into the C-8 position of purine residues in yeast tRNAPhe is shown to be markedly dependent on the conformation of the molecule. The completely unfolded molecule incorporates tritium at a rate commensurate to that expected for free purine nucleotides, but partially or completely folded forms incorporate proportionately less. The labeling of specific purine sites is determined by digesting the nucleic acid with a specific nuclease and analyzing each of the radioactive fragments produced. This analysis reveals that the amount of labeling of a purine in the folded form is strongly dependent upon its position in the sequence, whereas purine labeling in the unfolded form is independent of sequence position. The labeling pattern of the different bases in the folded form agrees fairly well with what is expected, based on existing solution and x-ray data on the conformation, i.e., residues involved in helical sections or apparently masked by tertiary interactions label more slowly than those on the "outside" of the molecule. Finally, folding and unfolding of specific regions of the macromolecule may be followed by the isotope labeling.
关键词：tritium labeling ; radioactive fingerprint analysis ; tertiary structure