文章基本信息

标题：Polyadenylylation and Transcription Following Fertilization
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作者：Isabel Slater ; D. W. Slater
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：4
页码：1103-1107
DOI：10.1073/pnas.71.4.1103
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Polyadenylylated RNA from sea urchin embryos concomitantly labeled with [3H]adenosine and [14C]uridine between fertilization and the four-cell stage was used to determine whether the RNA primers prerequisite to the massive polyadenylylation known to occur after fertilization are synthesized during oogenesis or subsequent to fertilization. Characterization of this RNA and unlabeled RNA via retention on nitrocellulose membranes and poly(U)-impregnated filters, molecular hybridization with [3H]poly(U), RNase resistance, oligo(dT)-cellulose chromatography, and size-distribution studies indicates that the poly(A) tracts synthesized after fertilization are predominantly appended to preexisting cytoplasmic primers of oogenic origin. Hence, if polyadenylylation is involved in the selective editing of presumptive genetic messages, this process is not confined to the nucleus unless a given codogenic transcript can undergo more than one cycle of adenylylation.
关键词：sea urchin embryos ; cytoplasmic adenylylation ; maternal and embryonic primers