文章基本信息

标题：Activity of Colicin E3-Treated Ribosomes in Initiation and in Chain Elongation
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作者：Phang-Cheng Tai ; Bernard D. Davis
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：4
页码：1021-1025
DOI：10.1073/pnas.71.4.1021
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Colicin E3 (called E3 here) is shown to act on polysomal ribosomes as well as on free ribosomes. This treatment severely inhibits subsequent chain elongation, both on preformed polysomes completing their nascent chains and on ribosomes translating poly(U). Elevated Mg++ concentration relieves this inhibition. However, elevated Mg++ concentration does not relieve the inhibition of ribosomes initiating on natural messenger; hence damage by E3 appears to have an additional, specific effect on some stage in initiation. The effect of damage by E3 resembles that of streptomycin in several respects. This similarity suggests that the two agents may act on closely related sites on the ribosome.
关键词：purified polysomes ; immunity substance ; streptomycin