文章基本信息

标题：A Functional Mitotic Spindle Prepared from Mammalian Cells in Culture
本地全文：下载
作者：W. Zacheus Cande ; Judith Snyder ; Diana Smith 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：4
页码：1559-1563
DOI：10.1073/pnas.71.4.1559
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mitotic cells lysed into solutions of polymerizable microtubule protein contain a spindle which is similar to the living spindle in two respects: it will lose and gain birefringence when cooled and warmed, and it will move anaphase chromosomes to the opposite ends of the cell. Early anaphase cells lysed into buffers containing high molecular weight polyethylene glycol and nucleotide triphosphates will continue chromosome motion and spindle elongation in the absence of exogenous spindle subunits. These results suggest that while spindle growth requires microtubule polymerization, anaphase motions do not.
关键词：microtubule assembly ; motility ; anaphase in vitro