文章基本信息

标题：Synthesis of Nerve Growth Factor by L and 3T3 Cells in Culture
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作者：Joel Oger ; Barry G. W. Arnason ; Nicholas Pantazis 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：4
页码：1554-1558
DOI：10.1073/pnas.71.4.1554
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mouse submaxillary gland nerve growth factor (NGF) has been covalently joined to bacteriophage and the resulting phage conjugates remain biologically active in stimulating neurite extension from sensory ganglia. A sensitive bacteriophage immunoassay has been developed to measure concentrations of NGF as low as 1 ng/ml. With this method, we find that mouse L and 3T3 cells in culture produce a biologically active nerve growth factor that is immunologically similar if not identical to mouse submaxillary gland NGF. Since L cells are known to be a source of "conditioned medium" for tissue culture, it could be that one or more of the conditioning factor activities secreted by these cells are due to NGF itself.
关键词：conditioned culture medium ; bacteriophage immunoassay ; fibroblasts