文章基本信息

标题：Evidence for Somatic Generation of Antibody Diversity
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作者：S. Tonegawa ; C. Steinberg ; S. Dube 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：4027-4031
DOI：10.1073/pnas.71.10.4027
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：RNA preparations containing 70-80% mouse {kappa}-chain mRNA have been prepared. The remainder consists of many RNA species, each of which represents a small fraction of the total RNA. The {kappa}-chain mRNA preparation hybridizes with mouse liver DNA with bi-phasic kinetics, indicating that it consists of two fractions --"unique" and "reiterated." Competition hybridization experiments show that the homology among the unique fractions from different mRNAs is the same as the homology among the amino acid sequences of the corresponding {kappa}-chains. Hence, in addition to the C-region (constant-region) sequences, (most of) the V-region (variable-region) sequences are also derived from unique germ line genes. The reiterated fractions from different {kappa}-chain mRNAs show essentially complete homology with each other. This fraction seems to consist mostly of sequences which do not code for amino-acid sequences of the secreted polypeptide chain, i.e., the "external" section of the mRNA molecule. It is concluded that the number of germ line genes is too small to account for the observed diversity of antibody molecules.
关键词：RNA·DNA hybridization ; κ-chain mRNA