文章基本信息

标题：Stereospecific Binding of Propranolol and Catecholamines to the β-Adrenergic Receptor
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作者：Daphne Atlas ; Michael L. Steer ; Alexander Levitzki 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：4246-4248
DOI：10.1073/pnas.71.10.4246
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The stereospecificity of the {beta}-adrenergic receptor of turkey erythrocyte ghosts was studied. Binding of the specific {beta}-adrenergic antagonist, propranolol, was found to occur exclusively with the l-stereoisomer. The stereospecificity of catecholamine binding was determined by assaying the ability of the catecholamines to displace [3H]propranolol. Binding of the catecholamines was also found to be stereospecific for the l-stereoisomers. Furthermore, the d-stereoisomers do not compete with the l form for binding. Using this displacement technique, we were able to calculate the dissociation constants for l-epinephrine, l-norepinephrine, and l-isoproterenol from the {beta}-receptor and compare these values to the apparent dissociation constants obtained from the direct activation of adenylate cyclase (EC 4.6.1.1 ) by these catecholamines.
关键词：stereospecificity ; erythrocyte